DiaPlexQ™ Entero Virus (Respiratory) Detection Kit
Real-Time OneStep RT-PCR based assay system for detection of enterovirus
CE-IVD
Enterovirus (EV) is positive sense single-strand RNA (+ssRNA) virus of icosahedral symmetry. Poliovirus, coxsackie A, B Virus and Eco Virus and Enterovirus are group of them. The virus firstly multiply in the mucosal cells from gastrointestinal tract, GI tract. And then show the pathogenic reaction through the central nervous system reaction and extend the infection. Additionally multiplied virus expose as a stool and being the one of infection factor. The symptom of Enterovirus is normally Shows similar as influenza or general respiratory illness, however, part of virus can be the cause of herpangina, aseptic meningitis, hand-foot- and mouth disease, myocarditis, acute hemorrhagic conjunctivitis virus. Rarely the virus infect to central nervous system and being the factor of encephalitis or encephalomeningitis. In a serious case it remains after effects or death of factor. * Please inquire us for compatible instrument information before use.
Specification
| DiaPlexQ™ Entero Virus (Respiratory) Detection Kit | |
|---|---|
| Detection target | Entero Virus (A/B/D type) |
| Registration | CE-IVD |
| Detection technology | Real-Time OneStep RT-PCR |
| Specimen type | Nasopharyngeal swab, Nasopharyngeal aspirate, Bronchoalveolar lavage (BAL), Throat swab, Nasal aspirates, Sputum |
| Analytical sensitivity | 10 copies |
| Compatible instruments* | ABI 7500 / 7500 Fast Real-Time PCR System (Applied Biosystems) CFX96™ Real-Time PCR System (Bio-Rad) |
| PCR running time | ~ 2 hrs |
Features
Features
- Simple & Rapid detection system : OneStep Multiplex qRT-PCR based detection
- HotStart PCR system : Ultra high specific and sensitive result
- Reliable system : Automatic PCR control
- Positive control included
Experimental
| Sample type | FAM | Texas Red / Cal Red 610 | Result |
|---|---|---|---|
| Positive Control | + | + | Valid |
| Negative Control | - | + | Valid |
| NTC (Non-Template Control) | - | + | Valid |
| Sample case 1 | + | + | Positive |
| Sample case 2 | - | + | Negative |
| Sample case 3 | + | - | Valid (Positive) |
| Sample case 4 | - | - | Required re-experiment |
Citation&Papers
Grist NR, Bell EJ, Assaad F. Enteroviruses in human disease. Prog Med Virol. 1978;24:114-57 | Muir P, Nicholson F, Illavia SJ, et al. Serological and molecular evidence of enterovirus infection in patients with endstage dilated cardiomyopathy. Heart. 1996;76(3):243-9. | Tebruegge M, Curtis N. Enterovirus infections in neonates. Semin Fetal Neonatal Med. 2009;14(4):222-7. | Imamura T1, Fuji N, Suzuki A, et al. Enterovirus 68 among children with severe acute respiratory infection, the Philippines. Emerg Infect Dis. 2011;17(8):1430-5. |
Ordering information
| Technology | Cat. No. | Product | Contents |
|---|---|---|---|
Real-Time PCR | SQD51-K020 (20 reaction) | DiaPlexQ ™ Entero Virus (Respiratory) Detection Kit | OneStep qRT-PCR Enzyme mix (Entero-Res.) 2X OneStep qRT-PCR Buffer (Entero-Res.) Primer & Probe Mixture (Entero-Res.) Control Template (Entero-Res.) RNase free Water |
| SQD51-K100 (100 reaction) |
