DiaPlexQ™ Novel Coronavirus (2019-nCoV) Detection Kit, Covid19, Corona virus
[SHT] Solg™ h-Taq DNA Polymerase
For High specific PCR amplification
Genotyping
Chemical-mediated Hot start enzyme
Ultra-high specificity
Amplicon size : ~ 1 kb
Solg™ h-Taq polymerase is chemically modified HotStart PCR enzyme, which enables multiplex and allele specific PCR for diagnostics and genotyping through initial heat activation during PCR cycling. You may modify the amount of template, extension time, annealing temperature, amount of Taq DNA polymerase, the number of PCR cycle, and amount of 5X Band Doctor™ according to the target size, primer’s Tm, and the type of templates for amplification.
Specification
| Source | Thermus aquaticus (Recombinant) | Amplicon size | ~ 1 kb |
| Hot start activity | Yes(Chemical-mediated) | ||
| 5’→ 3’ exonuclease activity | Yes | 3’A or Blunt ends | 3’A |
| 3’→ 5’ exonuclease activit y | No | Error rate | 12-13 bp error / 10⁶ bp |
Application
- Genotyping, Allele specific PCR, Highly specific amplification of genomic and cDNA targets, High G+C rich / Repeat region PCR, TA cloning
Citation&Papers
Lee GA, Kwon SJ, et al., Cross-amplification of SSR markers developed from Allium sativum to other Allium species, Scientia Horticulturae , 128 : 401-407, 2011 | Kee SK, Lee JY,
et al., Hypermethylation of the Ras Association Domain Family 1A (RASSF1A) Gene in Gallbladder Cancer, Molecules and Cells , 24 : 364-371, 2007 | Lee HJ, Cha KE, et al., In voitr Screening System for Hepatotoxicity: Comparison of Bone-Marrow-Derived Mesenchymal Stem Cells and Placenta-Derived Stem Cells,J ournal of Cellular Biochemistry, 112 : 49-58, 2011 |
Format
| Format | Product Image | Contents | Storage & Expiry Date |
|---|---|---|---|
| Standard |   | Solg ™ h-Taq DNA Polymerase (2.5 U/㎕) 10X h-Taq Reaction Buffer (15 mM MgCl₂mixed) 10 mM dNTP Mix 5X Band Doctor™ | 2 years 3 months (In case of Store at -20 ℃ ± 5 ℃) |
| Smart mix |   | 2X h-Taq PCR Smar t mix | 2 years 3 months (In case of Store at -20 ℃ ± 5 ℃) |
| Pre-Mix |   | 2X h-Taq PCR Pre-Mix | 1 year 6 months (In case of Store at -20 ℃ ± 5 ℃) |
Ordering information
| Format | Cat. No. | Product | Size | Price(won) |
|---|---|---|---|---|
| Standard | SHT06-R250 | Solg ™ h-Taq DNA Polymerase | 250 U (200 reaction) | - |
| SHT06-R10h | Solg ™ h-Taq DNA Polymerase | 1,000 U (250 U x 4 ea) | - | |
| SHT06-R25h | Solg ™ h-Taq DNA Polymerase | 2,500 U (250 U x 10 ea) | - | |
| SHT06-R50h | Solg ™ h-Taq DNA Polymerase | 5,000 U (250 U x 20 ea) | - | |
| Smart mix | SHT01-M40h | Solg ™ 2X h-Taq PCR Smar t mix 1, with dye | 1 ㎖ x 4 ea (160 reaction) | - |
| SHT03-M40h | Solg ™ 2X h-Taq PCR Smar t mix 2 (1.0X B and Doctor), with dye | 1 ㎖x 4 ea | - | |
| Pre-Mix | SHT01-P096 | Solg ™ 2X h-Taq PCR Pre-Mix, with dye, total volume 30 ㎕ reaction | 96 tube (8 strip x 12 ea) | - |
| SHT01-P288 | Solg ™ 2X h-Taq PCR Pre-Mix, with dye, total volume 30 ㎕ reaction | 288 tube (8 strip x 36 ea) | - | |
| SHT01-P576 | Solg ™ 2X h-Taq PCR Pre-Mix, with dye, total volume 30 ㎕ reaction | 576 tube (8 strip x 72 ea) | - | |
| Single item | SHT21-B50h | Solg ™ 10X h-Taq Reaction Buffer | 1 ㎖ x 5 ea (1,000 reaction) | - |
| SHT95-E250 | Solg™ h-Taq DNA Polymerase (only enzyme) | 250 U | - |
