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[DR] DiaStar™ OneStep RT-PCR Series (cDNA Synthesis)
Model Name
-
Series
Research Reagents (PCR)
Data
메뉴얼-솔젠트-DR31-M10h,_DR31-M50h,_DR32-M10h,_DR32-M50h,_DiaStar™_2X_OneStep_RT-PCR_Smart_mix
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SOLGENT

DiaPlexQ™ Novel Coronavirus (2019-nCoV) Detection Kit, Covid19, Corona virus

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Manufacturer
SOLGENT
Brand
-
SKU
125180
Product Name
[DR] DiaStar™ OneStep RT-PCR Series (cDNA Synthesis)
Model Name
-
Size
-
Weight
-
Product Details

[DR] DiaStar™ OneStep RT-PCR Series

For cDNA synthesis & PCR amplification at ‘One Step’ from RNA template


DiaStar™ OneStep RT-PCR Kit is a product for easy reverse transcription and PCR at “One Step” with optimal recombinant of DiaStarTM Reverse Transcriptase and Solg™ HotStart h-Taq DNA Polymerase mixture. This kit has dual advantages of high efficiency for cDNA synthesis and high specificity of hot start polymerase to set up of optimal PCR condition easily and conveniently



Specification

Reverse transcriptionSourceM-MLV (Recombinant)RNase H activityRNase H-
cDNA length~ 14 kb
Reaction temperature42 – 55 ℃Applicable PrimersGene-specific primer
PCRSourceThermus aquaticus (Solg™ h-Taq)Hot start activityYes (Chemical-mediated)
Amplicon size~ 1 kb3’A or Blunt ends3’A



Application

One-Step Multiplex RT-PCR, Gene expression analysis, RNA virus research, TA cloning




Citation&Papers

- Chung EY, Kim BH,

   et al., Resveratrol down-regulates interferon-γ-inducible inflammatory genes in macrophages: molecular mechanism via decreased STAT-1 

   activation, JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2010




Format

FormatProduct ImageContentsStorage & Expiry Date
Standard

DiaStar™ OneStep RT-PCR Enzyme Mix
5X OneStep RT-PCR Buffer
each 10 mM dNTP Mix
5X Band Doctor™
RNase-free water
1 year (In case of Store at -20 ℃ ± 5 ℃)
Smart mix

2X OneStep RT-PCR Smart mix1 year (In case of Store at -20 ℃ ± 5 ℃)
Pre-Mix

2X OneStep RT-PCR Pre-Mix1 year (In case of Store at -20 ℃ ± 5 ℃)




Ordering information

FormatCat. No.ProductSizePrice (won)
StandardDR61-K050DiaStar™ OneStep RT-PCR Kit50 reaction-
DR61-K100DiaStar™ OneStep RT-PCR Kit100 reaction-
Smart mixDR31-M10hDiaStar™ 2X OneStep RT-PCR Smart mix, with dye²1 ㎖
(66 reaction)¹
-
DR31-M50hDiaStar™ 2X OneStep RT-PCR Smart mix, with dye1 ㎖ x 5 ea-
Pre-MixDR31-P096DiaStar™ 2X OneStep RT-PCR Pre-Mix, with dye, total volume 30 ㎕ reaction96 tube
(8 strip x 12 ea)
-
Single itemDR65-B50hDiaStar™ 5X OneStep RT-PCR Reaction Buffer1 ㎖ x 5 ea
(833 reaction)
-



More products
SOLGENT
DiaPlexQ™ Novel Coronavirus (2019-nCoV) Detection Kit, Covid19, Corona virus
For the past 19 years, SolGent grown as a company specialized in human molecular diagnostics projects. In celebration of its 20th anniversary, the company announced its vision of a new start, “SolGent 2.0” and is growing as a bio leader in the 4th Industrial Revolution. Sturdily amassed genome analysis technology and molecular diagnostic technology have become the prominent foundation of the molecular diagnostic market, and SolGent supply products and services with the highest quality to more than 25 countries including domestic research institutes, examination centers, medical institutions and universities. In particular, as “precision medicine,” which analyzes genomes to prevent and predict diseases, becomes a new medical paradigm, SolGent develops and supplies various genome analysis services including for cancer, and expands and applies these services to agricultural products to build the AG-BIO platform. Based on its innovative technology, SolGent develops NADPH coenzyme fixed quantity measurement products DiaPlexQ™ Novel Coronavirus (2019-nCoV) Detection Kit, Covid19, Corona virus
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